Publications - Publications en-us PURE Extension (Web Department) 30 <![CDATA[Strong impact of nitrogen loading on submerged macrophytes and algae]]> Olsen, S., Chan, F., Li, W., Zhao, S., Søndergaard, M., Jeppesen, E. Excess loading of phosphorus (P) and nitrogen (N) triggers a shift in the trophic structure of shallow lakes from a clear-water, macrophyte-dominated state to an algal-dominated turbid state. However, the role of N in the shift is debated, and experimental evidence is, with a few exceptions, based on short-term studies (days to a few months). We studied the effect of N loading on macrophytes (dominated by Potamogeton lucens and Cabomba caroliniana), periphyton, filamentous algae and phytoplankton in mesocosms over 10 months (starting in October) in subtropical China (Wuhan). There were three N treatments: controls (CN) without nitrogen addition (mean TN = 1.9 mg L-1), low nitrogen (LN) addition (mean TN = 3.5 mg L-1) and high nitrogen (HN) addition (mean TN = 5.5 mg L-1). Total phosphorus (TP) concentration in the water column remained moderate (0.05-0.07 mg L-1) during the experiment in all treatments. Macrophyte abundance declined in the LN and HN treatments in the first 6 months, but not in controls, followed by a partial recovery in the LN treatments. They disappeared completely in the HN treatments the following summer. Periphyton (biofilm on plastic) and phytoplankton biomass remained unaffected during the first 6 months but increased over the summer by two or three times, compared with controls, in low and high nitrogen treatments, respectively. By contrast, the abundance of filamentous algae increased over winter but declined during the summer with no obvious relationship to the N treatments. There was no difference in the TN or nitrate concentrations or soluble protein, soluble sugar and Chl-a content of P. lucens leaves and stems with increasing N load. Macrophyte populations are partially resilient to abrupt increases in N loading at moderate TP concentrations, but, after prolonged exposure, a complete collapse occurs. Our results further indicate that macrophyte loss is exacerbated by shading by filamentous algae during the winter, and by phytoplankton and periphyton in the summer, while there was no indication of direct N toxicity.

Research Thu, 01 Jan 2015 22:18:15 +0100 312832e1-bf5f-4422-9cd2-7343d83898e4
<![CDATA[Response of Vallisneria spinulosa (Hydrocharitaceae) to contrasting nitrogen loadings in controlled lake mesocosms]]> Zhao, S., Yin, L., Chang, F., et al. The role of nitrogen (N) in the shift from a macrophyte-dominated state to a phytoplankton-dominated one at high N concentrations in shallow lakes is still debated. To elucidate possible toxic and ecological effects of high N on macrophyte growth, we conducted a short-term (40 day) study of a eutrophication-tolerant macrophyte, Vallisneria spinulosa (Hydrocharitaceae), incubated in pots in a mesocosm system subjected to different N concentrations (1, 3, and 5 mg l−1). Plant leaf and root length as well as growth rate decreased significantly with increased N concentrations, but most N- and P-related physiological parameters, including the soluble protein content, nitrate reductase activity, acid phosphatase activity, and tissue N and P contents, did not differ significantly among the N treatments. Only the alkaline phosphatase activity differed, being lower at high nitrogen loading, likely due to P limitation. Epiphyton and phytoplankton biomasses increased significantly with increasing N loading. Our results including a large number of physiological tests of the macrophytes, therefore, provide supporting evidence that the loss of submerged macrophytes, like V. spinulosa, seen at high N loading in shallow lakes, can be attributed to competition with phytoplankton and epiphyton rather than to toxic effects.

Research Mon, 01 Feb 2016 22:18:15 +0100 e537d275-dc44-4914-b82b-010a4d8d7411
<![CDATA[Impact of nutrients, temperatures, and a heat wave on zooplankton community structure]]> Işkın, U., Filiz, N., Cao, Y., et al. Shallow lakes are globally the most numerous water bodies and are sensitive to external perturbations, including eutrophication and climate change, which threaten their functioning. Extreme events, such as heat waves (HWs), are expected to become more frequent with global warming. To elucidate the effects of nutrients, warming, and HWs on zooplankton community structure, we conducted an experiment in 24 flow-through mesocosms (1.9 m in diameter, 1.0 m deep) imitating shallow lakes. The mesocosms have two nutrient levels (high (HN) and low (LN)) crossed with three temperature scenarios based on the Intergovernmental Panel on Climate Change (IPCC) projections of likely warming scenarios (unheated, A2, and A2 + 50%). The mesocosms had been running continuously with these treatments for 11 years prior to the HW simulation, which consisted of an additional 5 C increase in temperature applied from 1 July to 1 August 2014. The results showed that nutrient effects on the zooplankton community composition and abundance were greater than temperature effects for the period before, during, and after the HW. Before the HW, taxon richness was higher, and functional group diversity and evenness were lower in HN than in LN. We also found a lower biomass of large Cladocera and a lower zooplankton: phytoplankton ratio, indicating higher fish predation in HN than in LN. Concerning the temperature treatment, we found some indication of higher fish predation with warming in LN, but no clear effects in HN. There was a positive nutrient and warming interaction for the biomass of total zooplankton, large and small Copepoda, and the zooplankton: phytoplankton ratio during the HW, which was attributed to recorded HW-induced fish kill. The pattern after the HW largely followed the HW response. Our results suggest a strong nutrient effect on zooplankton, while the effect of temperature treatment and the 5 C HW was comparatively modest, and the changes likely largely reflected changes in predation.

Research Tue, 01 Dec 2020 22:18:15 +0100 ab938213-373d-40c4-bb18-a46792a88189
<![CDATA[Lake restoration]]> Søndergaard, M., Jeppesen, E., Jensen, H. S. Research Sun, 01 Jan 2012 22:18:15 +0100 323ecab3-78a6-4b4a-9d22-e2f018e27bb9 <![CDATA[Consistent stoichiometric long-term relationships between nutrients and chlorophyll-a across shallow lakes]]> Graeber, D., McCarthy, M. J., Shatwell, T., et al. Aquatic ecosystems are threatened by eutrophication from nutrient pollution. In lakes, eutrophication causes a plethora of deleterious effects, such as harmful algal blooms, fish kills and increased methane emissions. However, lake-specific responses to nutrient changes are highly variable, complicating eutrophication management. These lake-specific responses could result from short-term stochastic drivers overshadowing lake-independent, long-term relationships between phytoplankton and nutrients. Here, we show that strong stoichiometric long-term relationships exist between nutrients and chlorophyll a (Chla) for 5-year simple moving averages (SMA, median R² = 0.87) along a gradient of total nitrogen to total phosphorus (TN:TP) ratios. These stoichiometric relationships are consistent across 159 shallow lakes (defined as average depth < 6 m) from a cross-continental, open-access database. We calculate 5-year SMA residuals to assess short-term variability and find substantial short-term Chla variation which is weakly related to nutrient concentrations (median R² = 0.12). With shallow lakes representing 89% of the world’s lakes, the identified stoichiometric long-term relationships can globally improve quantitative nutrient management in both lakes and their catchments through a nutrient-ratio-based strategy.

Research Sun, 01 Dec 2024 22:18:15 +0100 38d6b759-21aa-48aa-abc9-01b137dd7eb2
<![CDATA[Benthic primary production decreases internal phosphorus loading from lake sediments under light supplement]]> Zhang, M., Li, Y., Uddin, K. B., et al. In aquatic ecosystems, light penetrating the sediment surface in shallow lakes may regulate the internal phosphorus (P) release through benthic primary production, which subsequently affects oxidation, pH levels, and alkaline phosphatase activity in the upper sediment. To study the effects of light exposure on the P dynamics at the sediment-water interface under eutrophic conditions, a two-month mesocosm experiment was conducted in twelve cement tanks (1000 L each). The tanks were equipped with Light-Emitting Diode (LED) lights, and surface sediments collected from eutrophic Lake Nanhu (China) were exposed to four different light intensities (0, 50, 100, 200 μmol m−2 s−1). The results revealed that: 1) Both the total phosphorus concentration and the phosphorus release flux from the sediment were lower in the light treatments (mean value, 0.59–0.71 mg L−1 and 0.00–0.01 mg m−2 d−1, respectively) than in the control treatment (0.77 mg L−1 and 0.01 mg m−2 d−1, respectively), indicating that light supplement could decrease the internal P release. 2) Benthic primary production promoted by light directly absorbed soluble reactive phosphorus and decreased the internal P release. The resulting improved production could also increase dissolved oxygen concentrations at the sediment-water interface, thus indirectly inhibiting internal P release. 3) The relative contributions of direct absorption and indirect inhibition on the internal P release ranged between 23% to 69% and 31% to 77% depending on the light intensity.

Research Mon, 15 Jan 2024 22:18:15 +0100 37f45588-6c25-4069-94c7-aed4af5e7373
<![CDATA[Notat vedrørende besvarelse af henvendelser under Miljø- og Klimateknologi 2023]]> Nørremark, M., Hansen, M. J., Kai, P., Børsting, C. F., Konnerup, D. Commissioned Fri, 23 Feb 2024 22:18:15 +0100 b461290b-8bb2-4bab-ba98-a7a9986225e9 <![CDATA[Physico-chemical, sensory and oxidative quality of butter from cows fed 3-nitrooxypropanol]]> Sirinayake Lokuge, G. M., Maigaard, M., Lund, P., et al. dairy cows on physico-chemical and sensory properties, as well as oxidative stability of butter. Fortyeight
Danish Holstein cows were randomly assigned to control diets or 3-NOP supplemented diets at
60 mg 3-NOP kg1 feed DM. Compared to control butter, 3-NOP butter had higher proportions of shortand
medium-chain fatty acids (FAs) and lower solid fat content, onset crystallization and offset melting
temperatures. Sensory analysis revealed minor differences between 3-NOP and control butter, while
peroxide values of 3-NOP butter was lower than control butter during storage of 12 weeks. In conclusion,
the alterations in FA composition by 3-NOP led to minor changes in physical properties but improved
oxidative stability of butter without major changes in sensory characteristics.]]>
Research Mon, 01 Jan 2024 22:18:15 +0100 4473d229-2b8b-4332-8e02-a1831158b74e
<![CDATA[Relation of early changes of QT dispersion to changes in left ventricular systolic and diastolic function after a first acute myocardial infarction]]> Møller, J. E., Husic, M., Søndergaard, E., Poulsen, S. H., Egstrup, K. OBJECTIVE: To describe the relation between changes of left ventricular systolic and diastolic function and changes of QT dispersion (difference in duration between longest and shortest QT interval) following acute myocardial infarction.

DESIGN: QT dispersion was determined at admission, hospital discharge, and 1 and 3 months following myocardial infarction in 64 consecutive 1-year survivors. Patients were divided into Group A where QT dispersion was < 52 ms at all recordings or initially > 52 ms but decreased during follow-up, and Group B where QT dispersion remained increased > or = 52 ms at all measurements. Doppler-Echocardiography was carried out on day 1, day 5, and after 1, 3, and 12 months.

RESULTS: In 26 patients QT dispersion remained increased > or = 52 ms during the first 3 months after infarction. Among these a significant increase of end-systolic volume was seen whereas low or rapid normalized QT dispersion was associated with a significant decrease of ventricular volumes. After 1 year end-systolic (70 +/- 32 ml vs 49 +/- 16 ml, p = 0.006) and end-diastolic volumes (138 +/- 41 ml vs 105 +/- 22 ml, p = 0.001) were higher in Group B. In a multivariate model Group B was significantly related to an increase of end-diastolic volume (p = 0.01). In Group A diastolic function improved in eight patients and in two it deteriorated, whereas improvement was seen in one patient and deterioration in nine patients from Group B (p < 0.01).

CONCLUSION: Following myocardial infarction low QT dispersion is associated with preserved left ventricular function, whereas persistently increased dispersion is associated with left ventricular dilation and deterioration of diastolic function.

Research Thu, 01 Aug 2002 22:18:15 +0200 a1b47525-3c5f-4b94-98f8-61caf6238137
<![CDATA[Impact of early changes in left ventricular filling pattern on long-term outcome after acute myocardial infarction]]> Møller, J. E., Poulsen, S. H., Søndergaard, E., Seward, J. B., Appleton, C. P., Egstrup, K. BACKGROUND: In patients with heart failure due to chronic ischemic heart disease improvement of diastolic function indicates improved survival and a reduced morbidity, but whether this is also the case after acute myocardial infarction is not known.

METHODS: To assess the prognostic importance of changes in left ventricular filling pattern, assessed with mitral deceleration time and colour M-mode flow propagation velocity, on cardiac death and readmission due to heart failure serial Doppler echocardiography was carried out in 103 patients with a first myocardial infarction. Based on echocardiography on hospital admission and after 1 month, patients were divided into three groups: group A (n=29) comprised patients with normal filling at either examination, group B (n=29) comprised patients with improvement of initially abnormal filling, and group C (n=45) patients with deterioration or no change of an abnormal filling pattern.

RESULTS: One-year survival free of cardiac death or hospitalisation for heart failure was 97% in group A, 86% in group B and 64% in group C (P<0.0001). In Cox analysis persistence of abnormal filling or deterioration of left ventricular filling was still a predictor of the combined endpoint (risk ratio 4.4, 95% CI 1.8-12.0, P=0.003) after adjustment of LV filling on admission, left ventricular systolic function and clinical variables. Serial analyses of left ventricular systolic function demonstrated a significant improvement after 1 year in ejection fraction in groups A and B, whereas ejection fraction remained unchanged in group C.

CONCLUSION: Patients with a persistently abnormal or a deterioration of left ventricular filling pattern as opposed to improved or normal filling are at increased risk of cardiac death and readmission due to heart failure after acute myocardial infarction.

Research Sun, 01 Jun 2003 22:18:15 +0200 50622c55-c075-4d74-a087-4103788a5b49
<![CDATA[Proteomics of the secretomes of bovine mammary epithelial cells]]> Che, J., Nielsen, S. D., Sattari, Z., et al. Research Mon, 22 Jan 2024 22:18:15 +0100 50d553f7-5780-4070-8af4-8cef8463f5d8 <![CDATA[Temporary stratification promotes large greenhouse gas emissions in a shallow eutrophic lake]]> Davidson, T. A., Søndergaard, M., Audet, J., et al. Shallow lakes and ponds undergo frequent temporary thermal stratification. How this affects greenhouse gas (GHG) emissions is moot, with both increased and reduced GHG emissions hypothesised. Here, weekly estimations of GHG emissions, over the growing season from May to September, were combined with temperature and oxygen profiles of an 11 ha temperate shallow lake to investigate how thermal stratification shapes GHG emissions. There were three main stratification periods with profound anoxia occurring in the bottom waters upon isolation from the atmosphere. Average diffusive emissions of methane (CH4) and nitrous oxide (N2O) were larger and more variable in the stratified phase, whereas carbon dioxide (CO2) was on average lower, though these differences were not statistically significant. In contrast, there was a significant order of magnitude increase in CH4 ebullition in the stratified phase. Furthermore, at the end of the period of stratification, there was a large efflux of CH4 and CO2 as the lake mixed. Two relatively isolated turnover events were estimated to have released the majority of the CH4 emitted between May and September. These results demonstrate how stratification patterns can shape GHG emissions and highlight the role of turnover emissions and the need for high-frequency measurements of GHG emissions, which are required to accurately characterise emissions, particularly from temporarily stratifying lakes.

Research Wed, 03 Jan 2024 22:18:15 +0100 6be40b01-c033-4cc4-a247-1ab89452539b
<![CDATA[Velfærdsvurdering af dyr på afstand –vurdering af indikatorer og tekniske hjælpemidler]]> Henriksen, B. I. F., Christensen, J. W. Commissioned Mon, 19 Feb 2024 22:18:15 +0100 feaf783b-8336-4f88-ad65-ad0314ded731 <![CDATA[Fire veterinær-professorer holdt tiltrædelsesforelæsning]]> Lærke, H. N. Communication Thu, 08 Feb 2024 22:18:15 +0100 d6a8ccab-3cd0-421d-8f89-3d52137bdb29 <![CDATA[Re]]> Hatch, E. E., Willis, S. K., Wesselink, A. K., et al. Research Mon, 01 Nov 2021 22:18:15 +0100 d89a5e1d-8940-4f2f-97b6-174b75043d49 <![CDATA[Weight at birth and subsequent fecundability]]> Wildenschild, C., Riis, A. H., Ehrenstein, V., et al. Objective: To examine the association between a woman's birth weight and her subsequent fecundability. Method: In this prospective cohort study, we included 2,773 Danish pregnancy planners enrolled in the internet-based cohort study ''Snart-Gravid'', conducted during 2007-2012. Participants were 18-40 years old at study entry, attempting to conceive, and were not receiving fertility treatment. Data on weight at birth were obtained from the Danish Medical Birth Registry and categorized as ,2,500 grams, 2,500-2,999 grams, 3,000-3,999 grams, and $4,000 grams. In additional analyses, birth weight was categorized according to z-scores for each gestational week at birth. Time-to-pregnancy measured in cycles was used to compute fecundability ratios (FR) and 95% confidence intervals (CI), using a proportional probabilities regression model. Results: Relative to women with a birth weight of 3,000-3,999 grams, FRs adjusted for gestational age, year of birth, and maternal socio-demographic and medical factors were 0.99 (95% CI: 0.73;1.34), 0.99 (95% CI: 0.87;1.12), and 1.08 (95% CI: 0.94;1.24) for birth weight ,2,500 grams, 2,500-2,999 grams, and $4,000 grams, respectively. Estimates remained unchanged after further adjustment for markers of the participant's mother's fecundability. We obtained similar results when we restricted to women who were born at term, and to women who had attempted to conceive for a maximum of 6 cycles before study entry. Results remained similar when we estimated FRs according to z-scores of birth weight. Conclusion: Our results indicate that birth weight appears not to be an important determinant of fecundability.

Research Tue, 15 Apr 2014 22:18:15 +0200 113d6c3a-d693-4e72-acf7-abdec89cd146
<![CDATA[Slagtesvin udskiller mere zink end nyfravænnede grise]]> Hansen, S., Nielsen, T. S. Communication Sat, 01 Jan 2022 22:18:15 +0100 5dd386e4-cadd-4b71-8b27-0158ef58f0c5 <![CDATA[Determination of the optimal dietary zinc content for pigs between 10 and 30 kg body weight]]> Hansen, S. V., Graffagnino, A., Hedemann, M. S., Nielsen, T. S., Woyengo, T. A. The Zn requirement of pigs immediately after weaning is more investigated compared to the Zn requirement in the growth period between 10 and 30 kg. Unabsorbed and excessive dietary Zn is excreted mainly through feces, and spreading pig slurry to fields can cause environmental issues because high levels of Zn can impair plant growth and contribute to the development of antimicrobial resistance genes in microorganisms. Therefore, more precise knowledge of Zn requirements and dietary Zn recommendations is important. The present study investigated the optimal dietary Zn content for 10- to 30-kg pigs. The study used 150 pigs weaned at 28 d of age (day 0) and supplied with 1,474 mg dietary Zn/ kg the first 2 wk post-weaning. After 2 wk, pigs were randomly distributed according to body weight (BW; 10.1 ± 0.3 kg) and sex, to individually housing, and fed a diet supplemented with either 0, 30, 60, 120, or 240 mg Zn/kg (from ZnO), resulting in total dietary Zn contents of 80, 92, 117, 189, and 318 mg/kg until week 6 post-weaning. BW, feed intake, and fecal scores were recorded, and samples of blood (weeks 2, 3, 5, and 6) and tissues (week 6) were collected. The feed intake, growth, feed efficiency, relative weight of the pancreas and liver, Zn concentration in the liver, and pancreatic digestive enzyme activity were unaffected by dietary Zn content (P > 0.12). The serum Zn level decreased (P < 0.01) by up to 24% from weeks 2 to 3. The serum Zn concentrations in weeks 5 and 6 were similar to in week 2 when 117, 189, and 318 mg Zn/kg were provided, while with 80 and 92 mg Zn/kg the serum Zn concentration was lower (P < 0.01) than in week 2. The serum Zn concentration reached a plateau in weeks 5 and 6, and breakpoints were calculated at 126 ± 17 and 102 ± 6 mg Zn/kg, respectively. Bone Zn status was greater (P < 0.01) with 189 than 80 mg Zn/kg and a breakpoint was calculated at 137 ± 19 mg Zn/kg. According to performance, the Zn requirement for 10- to 30-kg pigs can be fulfilled with 80 mg total Zn/kg, but based on serum and bone Zn status, the optimal total dietary Zn content is 102 to 137 mg/kg. The latter corresponds to a daily Zn intake (requirement) of 103 to 138 mg when calculated from the average feed intake during weeks 3 to 6 (1,005 g/d). Importantly, the presented results are obtained in pigs supplied with 1,474 mg Zn/kg from ZnO the first 2 wk post-weaning and a high level of phytase (1,000 phytase units) in the diet throughout the experiment.

Research Sun, 01 Jan 2023 22:18:15 +0100 577a28d8-f982-42d3-8f9a-6b7efab29bb9
<![CDATA[Vidensyntese om adfærdsmæssige indikatorer for hhv. positiv og negativ velfærd hos hest og kvæg ved helårsafgræsning]]> Jensen, M. B., Christensen, J. W. Commissioned Mon, 05 Feb 2024 22:18:15 +0100 122a40ff-9f46-4b21-81a8-7a306c81bdcc <![CDATA[”Jul i det gamle kalveskjul”, video]]> Communication Thu, 21 Dec 2023 22:18:15 +0100 ef0a8a3b-9720-4121-8004-bc63bfbc801b <![CDATA[Systematisk sygdomsforebyggelse i kalveopdrættet, SEGES TV 29.03.23]]> Communication Wed, 29 Mar 2023 22:18:15 +0200 e8775d5a-9855-4258-8cfa-8e063916625c <![CDATA[Genome-wide association study and functional annotation analyses for nitrogen efficiency index and its composition traits in dairy cattle]]> Chen, Y., Atashi, H., Grelet, C., et al. Research Mon, 01 May 2023 22:18:15 +0200 98235689-f04b-4139-8578-0b951f821411 <![CDATA[Validating genomic prediction for nitrogen efficiency index and its composition traits of Holstein cows in early lactation]]> Chen, Y., Atashi, H., Mota, R. R., et al. Research Wed, 01 Nov 2023 22:18:15 +0100 5a0faee2-3df5-4658-9b7f-c067b5a5631c <![CDATA[An organism-wide ATAC-seq peak catalog for the bovine and its use to identify regulatory variants]]> Yuan, C., Tang, L., Lopdell, T., et al. cis-acting regulatory elements for the bovine detected by the assay for transposase accessible chromatin using sequencing (ATAC-seq). We regroup these regulatory elements in 16 components by nonnegative matrix factorization. Correlation between the genome-wide density of peaks and transcription start sites, correlation between peak accessibility and expression of neighboring genes, and enrichment in transcription factor binding motifs support their regulatory potential. Using a previously established catalog of 12,736,643 variants, we show that the proportion of single-nucleotide polymorphisms mapping to ATAC-seq peaks is higher than expected and that this is owing to an approximately 1.3-fold higher mutation rate within peaks. Their site frequency spectrum indicates that variants in ATAC-seq peaks are subject to purifying selection. We generate eQTL data sets for liver and blood and show that variants that drive eQTL fall into liver- and blood-specific ATAC-seq peaks more often than expected by chance. We combine ATAC-seq and eQTL data to estimate that the proportion of regulatory variants mapping to ATAC-seq peaks is approximately one in three and that the proportion of variants mapping to ATAC-seq peaks that are regulatory is approximately one in 25. We discuss the implication of these findings on the utility of ATAC-seq information to improve the accuracy of genomic selection. ]]> Research Sun, 01 Oct 2023 22:18:15 +0200 0171e20d-6570-4711-b0eb-870fe561f6d4 <![CDATA[Welfare Assessments of Broiler Chickens - Exploring Confounding Factors]]> Rasmussen, S. Research Mon, 01 Jan 2024 22:18:15 +0100 ce72e087-1f32-4cf7-bcde-03175ff2d012 <![CDATA[A practical guide to paired inspection]]> Mc Loughlin, E. T. In order to meaningfully detail the benefits, challenges, and practical recommendations, this paper draws ona wide range of literature but crucially gained insights from inspectors through an online survey that was distributed through my own and the associated EURCAW-Pigs network. The survey included 15 questionsand was distributed to 40 inspectors. 18 completed the survey, a further 4 started but did not complete. The aim of the survey is to gather insights from their experiences, the success of pair inspection, and any challenges they have experienced. It is not designed to be a representative sample of European inspectors as this was not possible within the timeframe, but an information-gathering exercise to identify advantages, disadvantages, and unforeseen aspects of paired inspection that may be possibly lacking from the literature
on paired inspection.]]>
Research Mon, 08 Jan 2024 22:18:15 +0100 a10ab5e1-7c71-460a-b84b-0dd4eea48fc3
<![CDATA[Giv sundhedsstyringen i kalveholdet et skub med få enkle redskaber]]> Myhlendorph-Jarltoft, T., Martin, H. L. i den enkelte besætning. Der findes gode, let tilgængelige redskaber. Her kommer
en opsamling på de vigtigste.]]>
Communication Sun, 01 Jan 2023 22:18:15 +0100 206ccc31-bd1a-402a-b959-ceb4b5def9cb
<![CDATA[Prediction of mortality in land-based recirculating aquaculture system (RAS) trout farms]]> Foldager, L., Thorup, V. M., Pastell, M. ., Jensen, B. B., Krogh, M. A. 2 and dissolved oxygen (DO; amount of oxygen accessible to the fish) on basis of time series sensor data, and the use of generalised dynamic linear models.]]> Research Mon, 15 Jan 2024 22:18:15 +0100 51a99d33-eaec-4808-b3b1-d9e66ccaebfd <![CDATA[The recovery rate of methane, carbon dioxide and hydrogen at different flow levels in respiration chambers]]> Hellwing, A. L. F., Weisbjerg, M. R., Lund, P. Research Tue, 06 Aug 2019 22:18:15 +0200 e50027b7-d4ae-4843-9e8a-16442e26c207 <![CDATA[CO2 and CH4 Emissions from Manure in Respiration Chambers are Neglible Compared to Cow´s Emissions]]> Hellwing, A. L. F., Maigaard, M., Nielsen, M. O., et al. Research Mon, 06 Jun 2022 22:18:15 +0200 3b9e6823-f06c-4179-8462-35bd118b640c <![CDATA[Opdatering af baseline 2027]]> Andersen, H. E., Børsting, C. F., Christensen, J. H., et al. Commissioned Sun, 01 Jan 2023 22:18:15 +0100 37d1c09e-6349-4d2a-bcec-32d0a7374bdf <![CDATA[Når ulykken er ude - dyrevelfærd under beredskabsindsats efter uheld med dyretransporter]]> Kobek-Kjeldager, C., Dahl-Pedersen, K., Herskin, M. S. Commissioned Wed, 17 Jan 2024 22:18:15 +0100 4ba3a9aa-49ca-4af6-8c2e-23ad7a6c8307 <![CDATA[Gennemgang af bioakustiske værktøjers potentiale til at vurdere landbrugsdyrs sundhed og velfærd]]> Coutant, M. P. Commissioned Wed, 17 Jan 2024 22:18:15 +0100 517522cb-f412-428a-99fe-90e122630910 <![CDATA[In vitro gas production of pre-treated or untreated feedstuffs using cecum inoculum from horses]]> Jensen, R. H., Jensen, R. B., Nielsen, M. O., Hellwing, A. L. F. Research Sat, 26 Aug 2023 22:18:15 +0200 4c14cc06-d95a-4a80-9898-08dfe2e88a68 <![CDATA[Climate beef – by more beef based on the dairy cow]]> Kargo, M. ., Buitenhuis, A. J., Kristensen, T., et al. Research Sun, 01 Jan 2023 22:18:15 +0100 e7c8e222-4113-49f9-af39-793a7ab45451 <![CDATA[Treatment of shoulder ulcers in sows - rubber mats and zinc ointment compared to chlortetracycline spray]]> Kaiser, M., Kristensen, C. S., Bækbo, P., Alban, L. BACKGROUND: Shoulder ulcers can have a significant impact on the welfare of sows. In Denmark, rubber mats are used for treatment of shoulder ulcers. The objective of the study was to compare a treatment consisting of a combination of rubber mats and zinc ointment with the effect of local antibiotic spray (chlortetracycline) on shoulder ulcers.

METHODS: A total of 304 sows with shoulder ulcers were observed shortly after farrowing (day 1) and on days 14 and 21 after the first observation. The sows were paired according to the grade of the shoulder ulcer using the official Danish scoring system. From each pair of sows, one sow was allocated to mat group (M) and the other to an antibiotic group (A) in a random way. In the M group, rubber mats were placed on the floor, and the ulcers were treated with zinc ointment once a day. In the A group, sows were treated with antibiotic spray daily. The size of the shoulder ulcer was measured manually on a continuous scale on days 1, 14 and 21. The data were analysed by use of two multivariable models where the response was the area of the shoulder ulcer on day 14 and day 21, respectively. Explanatory variables were treatment, herd, parity, body condition and size of ulcer on day 1. If a sow had an ulcer on both shoulders, the shoulder with the largest ulcer was selected.

RESULTS: The treatment consisting of mats and zinc ointment had a statistical significant effect on the size of the shoulder ulcer on day 14 and day 21 compared to daily treatment with antibiotic spray. For lean sows that were kept on rubber mats and zinc ointment, the average shoulder ulcer was significantly smaller on day 14 (3.8 cm(2) versus 9.5 cm(2) when antibiotic spray was used) and day 21 (3.4 cm(2) versus 6.6 cm(2)) compared to lean sows that were only treated with antibiotic spray. For fat sows this was also the case when using the lesion on day 21 as the response (2.0 cm(2) versus 5.7 cm(2)) but not on day 14. Moreover, the size of the lesion on day 1 was statistically associated with the size of the lesion on day 14 and on day 21. The treatment was equally effective in the three herds.

CONCLUSIONS: Rubber mats and daily smearing with zinc ointment slow progression and contribute to the healing of shoulder ulcers compared with housing on slats and daily spraying with antibiotics. It is recommended to place a rubber mat in the farrowing crate at the first sign of shoulder ulcers.

Research Fri, 15 Feb 2013 22:18:15 +0100 c6750436-75e5-4375-8551-c3555c7eb7c4
<![CDATA[Grading of shoulder ulcerations in sows by biopsies]]> Jensen, H. E., Dahl-Pedersen, K., Barington, K., et al. Shoulder ulcerations can be graded postmortem from 0 to 4 on a pathoanatomical scale. However, veterinarians and farmers express difficulties evaluating the grade of the lesions antemortem. Accurate grading is needed in order to comply with veterinary instruction in relation to the Danish legislation, stating that sows with shoulder ulcers grade 3 or 4 must be kept loose and have access to soft bedding. Thus, the aim of the present study was to evaluate if biopsies from the center of a shoulder ulcer can be used to point out animals for which an intervention must be initiated. Postmortem, a punch biopsy was sampled from the center of the ulceration or from the tissue overlaying the tuber spina scapula. Afterward, the shoulders were cross-sectioned and evaluated grossly and histologically ("gold standard"). In total, 121 shoulders were included in the study, and the diagnostic value of a punch biopsy in grading shoulder ulcerations was evaluated. The results showed a sensitivity of 0.78, a specificity of 0.98, a positive likelihood ratio of 38.36, and a negative likelihood ratio of 0.22. The agreement between the cross-section evaluation and the punch biopsy was found to be 0.90 by calculating the Cohen kappa value. In conclusion, a single punch biopsy from the center of an ulcer is useful for determining the grade of a shoulder ulcer and can be used to facilitate the identification of sows with ulcers requiring an intervention.

Research Sat, 01 Mar 2014 22:18:15 +0100 a5808540-9e97-4352-bc5d-a32791b5578b
<![CDATA[Reproductive health of the sow during puerperium]]> Björkman, S., Kauffold, J., Kaiser, M. Ø. The modern hyperprolific sow is susceptible to metabolic disease and chronic inflammation. The most sensitive phase is parturition, when the sow experiences systemic inflammation and stress, and major changes in metabolism and endocrinology. Resolution of inflammation and stress needs to happen quickly to ensure good reproductive health during puerperium. If the sow fails to adapt to these changes, puerperal disease may occur. The economically most important puerperal disease complex is the postpartum dysgalactia syndrome (PPDS). Other puerperal diseases include infections of the urogenital tract. Diagnosis of PPDS and urogenital disease on-farm is challenging but several diagnostic methods, including clinical examination, behavioral observations, ultrasonography and biomarkers are available. Ultrasonography is an excellent tool for monitoring the health of the urogenital tract, the mammary gland, and uterine involution and guide further diagnostic interventions. Biomarkers such as Chromogranin A, tumor necrosis factor-α, and interleukin-6 represent promising tools to monitor general health and the systemic state of inflammation and oxidative stress of the sow. Nonsteroidal anti-inflammatory drugs, dopamine antagonists, and oxytocin are promising to address the symptoms of PPDS. Reducing of stress, improving nutrition and intestinal health, and supporting animal welfare-friendly husbandry help in the prevention of PPDS.

Research Sat, 01 Jul 2023 22:18:15 +0200 1b3dff91-f4f0-4b0f-854b-3d55e491f227
<![CDATA[Correction to]]> Kaiser, M., Jacobson, M., Andersen, P. H., et al. The original article [1] contains an error whereby the caption in Figure 8 is incorrect; the correct caption can be seen ahead alongside its respective image.

Research Fri, 01 Jun 2018 22:18:15 +0200 5db46ea9-393e-498e-aab9-d326259256ad
<![CDATA[Inflammatory markers before and after farrowing in healthy sows and in sows affected with postpartum dysgalactia syndrome]]> Kaiser, M., Jacobson, M., Andersen, P. H., et al. BACKGROUND: The pathogenesis of postpartum dysgalactia syndrome (PDS) in sows is not fully elucidated and affected sows often present vague clinical signs. Accurate and timely diagnosis is difficult, and PDS is often recognized with a delay once piglets begin to starve. Increased rectal temperature of the sow is an important diagnostic parameter, but it may also be influenced by a number of other parameters and is thus difficult to interpret. Inflammatory markers may be important adjuncts to the clinical assessment of sows with PDS, but such markers have only been studied to a limited extent. The objective was to characterize the inflammatory response in healthy sows and in sows suffering from PDS, and to identify biomarkers that may assist in early identification of PDS-affected sows.

RESULTS: Thirty-eight PDS-affected (PDS+) and 38 healthy (PDS-) sows underwent clinical examination and blood sampling every 24 h, from 60 h before the first piglet was born to 36 h after parturition. In both groups, inflammatory markers changed in relation to parturition. Most inflammatory markers changed 12-36 h after parturition [white blood cell counts (WBC), neutrophil counts, lymphocyte counts, tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), serum amyloid A (SAA), C-reactive protein (CRP), haptoglobin (Hp), iron (Fe) and albumin (ALB)]. Changes in neutrophil counts, lymphocyte counts, CRP, Fe and ALB were observed -12 to 0 h before parturition. WBC, neutrophil and lymphocyte counts, serum concentrations of TNF-α, IL-6, Hp and Fe differed between PDS+ and PDS- sows. These differences were mainly apparent 12 to 36 h after parturition, but already at 12 h before parturition, PDS+ sows had lower lymphocyte counts than PDS- sows.

CONCLUSIONS: Parturition itself caused significant inflammatory changes, but PDS+ sows showed a more severe response than PDS- sows. WBC, neutrophil and lymphocyte counts, and concentrations of TNF-α, IL-6, Hp and Fe can be potential biomarkers for PDS. Lymphocyte counts may be used to detect PDS at pre-partum. To assess their diagnostic potential, these markers must be investigated further and most likely combined with assessment of clinical parameters and other biomarkers for improved identification of sows at risk of developing PDS.

Research Mon, 12 Mar 2018 22:18:15 +0100 4dfd95a2-138d-4cdf-b583-743070964fb9
<![CDATA[Hormonal and metabolic indicators before and after farrowing in sows affected with postpartum dysgalactia syndrome]]> Kaiser, M., Jacobsen, S., Andersen, P. H., et al. BACKGROUND: Postpartum dysgalactia syndrome (PDS) in sows is difficult to diagnose and the pathogenesis is obscure. Hormonal changes related to the disease are often difficult to distinguish from those found in the normal transition period from gestation to lactation. The study aimed to investigate metabolic and hormonal changes related to PDS with the goal of identifying potential biomarkers in sows suffering from PDS (PDS+). Selected biomarkers were examined by comparing 38 PDS+ sows with 38 PDS negative (PDS-) sows. The sows were sampled every 24 h from 60 h ante partum (a.p.) to 36 h post partum (p.p.).

RESULTS: Compared to the baseline (60 to 36 h a.p.), cortisol in serum and saliva and fasting blood glucose concentrations increased in PDS+ as well as PDS- sows. C-peptide decreased relative to the baseline in PDS+ sows, and prolactin and 8-epi prostaglandin F2 alpha (8-epi-PGF2α) decreased in PDS- sows. Concentrations of cortisol in serum and saliva, salivary chromogranin A (CgA), fasting blood glucose, C-peptide, and 8-epi-PGF2α differed significantly between PDS+ and PDS- sows, with levels of cortisol in serum and saliva, salivary CgA, and 8-epi-PGF2α in serum being different in the two groups already before parturition. Concentrations of salivary CgA were significantly lower in PDS- sows than in PDS+ sows during the entire study period.

CONCLUSIONS: The results suggest that salivary CgA, cortisol and serum 8-epi-PGF2α may potentially serve as early diagnostic indicators for PDS. The consistently higher salivary CgA concentration in PDS+ sows compared to PDS- sows may indicate that homeostatic disturbances are present between 36 to 60 h before parturition in sows developing PDS. The higher serum and saliva cortisol concentration in PDS+ sows compared to PDS- sows could reflect an early sign of inflammation or stress. The significantly lower C-peptide in PDS+ sows compared to PDS- sows may reflect a lower food intake. Our results contribute to the understanding of the pathogenesis of PDS, and the homeostatic disturbances detected before parturition warrants further investigation. The diagnostic potential of the markers identified in this study should be investigated further in a larger population of sows.

Research Wed, 07 Nov 2018 22:18:15 +0100 ac9f9977-b530-4af9-a566-8ad3aa86ce62
<![CDATA[Dam Rearing of Dairy Calves - The Effect of Duration of Cow-calf-contact on the Strength of the Maternal Bond]]> Jensen, E. H. This PhD project is comprised three studies, which were all part of the same large experiment. Eighty-four cow-calf pairs were enrolled and allocated to one of three CCC treatments: full-time contact (23 h contact/d, only separated when cows were milked in the parlour); part-time contact (10 h contact/d, separated between afternoon and morning milking); or no contact (0 h contact/d, separated 48 h postpartum). These treatments were maintained for seven weeks (the main rearing period) and followed by period of reduced contact for two weeks. In the reduced contact period, half of full- and part-time cow-calf pairs experienced a gradual reduction in daily contact (reduced contact, RC). In the first week of the reduced contact period, the daily contact of RC pairs was reduced to approximately 50% of their original contact (9.5 h/d for full-time, 4.5 h/d for part-time). In the second week, contact was reduced even further to approximately 25% of the original amount (4.5 h/d for full-time, 2 h/d for part-time). The remaining cow-calf pairs maintained the same amount of contact as the first seven weeks (unchanged contact). The RC treatment was implemented as a way of gradually reducing nursing/suckling opportunity and was therefore expected to increase the calves’ nutritional independence, preparing them for weaning. In the tenth week of the experiment, all cows and calves were completely separated.
In Study I, the maternal behaviour of full- and part-time cows was observed for 24 h in the third week of the main rearing period, and the suckling behaviour of their calves was observed twice for 24 h during the reduced contact period; once following each reduction in CCC. In Study II and III, the cows’ motivations were assessed; both times using the maximum price paid (MPP) method. Cows were trained to pass through a push gate to reach their calf. The gate became increasingly heavier with every successful passing, and when the cow two days in a row failed to reach her calf, she was recorded as having reached her maximum price. The higher maximum price paid, the stronger the motivation. In Study II, the strength of the cows’ maternal bond was assessed, and in Study III, cows’ motivation to nurse was assessed. The maternal bond strength was measured during the main rearing period, while nursing motivation was measured during the reduced contact period. As there is a risk of animals becoming frustrated during MPP tests, cows always had the option of an easy-available, but less preferred alternative to the valued resource; in the maternal bond test, an unfamiliar calf was available as alternative to the cow’s own calf, and in the nursing motivation test, partial calf contact, where nursing was prevented, was available as an alternative to full contact, where nursing was possible. A secondary aim of the present PhD thesis was to appraise the effect of providing these alternatives in the MPP test.
In Study I, full-time cows spent more time nursing and grooming their calves than part-time cows did; however, no difference could be found regarding amount of nursing taking place in inverse parallel position nor the likelihood of a cow nursing an unrelated calf. These last two behaviours have been suggested as proxies for maternal bond strength. The observations on the calves revealed that reduced contact did not consistently reduce suckling time of neither full- or part-time calves. In Study II, no difference in maternal motivation was found between full- and part-time cows, while no-contact cows were significantly less maternally motivated. In Study III, reduced contact increased cows’ motivation to nurse compared to cows with unchanged calf contact (significantly for full-time, numerically for part-time). The alternative options provided in both motivation tests were used less than expected by random.
These results indicate, that even though part-time cows spent less time nursing their calves than full-time cows did, no difference was found in the strength of the maternal bonds formed. This finding may indicate that part-time contact is a good alternative to full-time contact, as it allows cows to be with their calves, which the motivation tests shows is highly important to them. On the other hand, the repeated nightly separation may be stressful to both cow and calf. The aim of the reduced contact period was to reduce calves’ suckling time and make them less dependent on their dam, which was expected to reduce the cows’ motivation to nurse. However, calves with reduced contact appeared able to compensate and maintain some suckling time. Additionally, cows with reduced calf contact were more motivated to nurse their calf than cows with unchanged contact. The gradual reduction was therefore unsuccessful in inducing weaning, instead cows were even more motivated to nurse their calves. This could be due to the age of the calves; the reduced contact period was initiated when calves were around 60 days old, which is much earlier than the expected natural weaning of 6 to 11 months, and the cows may simply not have had incentive to wean their calves at this point in time. Neither an unfamiliar calf nor partial contact appeared able to substitute own calf or ability to nurse, respectively. Further research on the welfare implications of part-time CCC and how to better induce weaning in CCC systems is therefore needed. Additionally, other methods to reduce potential frustration at testing should be investigated.]]>
Research Mon, 01 Jan 2024 22:18:15 +0100 07cd909a-5b1c-4d91-aff2-52fb58d0c721
<![CDATA[Optimal level of dietary zinc for pigs between 10 and 30 kg]]> Nielsen, T. S., Hansen, S., Nørgaard, J. V., Woyengo, T. A. Research Tue, 01 Aug 2023 22:18:15 +0200 57854c89-b3db-4be0-86c1-1029c230afd2 <![CDATA[Changes in piglet and litter characteristics across parities in two highly prolific sow hybrids in an outdoor organic herd]]> Kobek-Kjeldager, C., Larsen, M. L. V., Pedersen, L. J. Research Fri, 01 Dec 2023 22:18:15 +0100 584f503d-f5f6-4293-b91b-0f8a31a49540 <![CDATA[Effect of micronization and vitamin E supplementation on ruminal biohydrogenation kinetic of whole flaked rapeseed]]> Zarnegar, Z., Lashkari, S., Ebrahimi, S. H., Valizadeh, R., Jensen, S. K., Naserian, A. A. Research Mon, 01 Jan 2024 22:18:15 +0100 c12c86cc-c2c4-4ddf-8ac9-136f350ae575 <![CDATA[Næringsstofudskillelse fra får og geder, ab dyr]]> Lund, P. Commissioned Fri, 01 Dec 2023 22:18:15 +0100 71481a99-183b-4423-8bac-e63859eb5a03 <![CDATA[Næringsstofudskillelse fra pelsdyr, ab dyr]]> Møller, S. H. Commissioned Fri, 01 Dec 2023 22:18:15 +0100 327dccd5-61c0-4062-8478-7c13064daa78 <![CDATA[Næringsstofudskillelse fra fjerkræ, ab dyr]]> van der Heide, M. E., Børsting, C. F., Hellwing, A. L. F., Jensen, . H. B. Commissioned Fri, 01 Dec 2023 22:18:15 +0100 163c21d9-097c-423b-a44f-9861aacb8ed3 <![CDATA[Næringsstofudskillelse fra svin, ab dyr]]> Sørensen, M. T., Tybirk, P., Krogh, U. P., Hellwing, A. L. F., Børsting, C. F. Commissioned Fri, 01 Dec 2023 22:18:15 +0100 42df4646-ba26-49d9-9368-b3006df7b6e1 <![CDATA[Normtal 2023/2024]]> Commissioned Tue, 01 Aug 2023 22:18:15 +0200 d7b58579-3488-4ec2-943c-af54d488aef2