This project will be the first to identify metabolites in blood and urine, which can be used as physiological markers for branched-chain amino acid (BCAA) imbalances in pigs. Pig diets are supplemented with crystalline amino acids (AA) to reduce crude protein concentration and nitrogen excretion.
The BCAA is an interesting group of AA because of interactions in their catabolic pathways, and because they very often are limiting for a further reductions in dietary crude protein in pig diets. Presently, AA requirement is estimated by long-term measurements on growth and feed intake in dose-response experiments. The ideal physiological marker is a metabolite expressed in blood or urine in situations of deficient supply of dietary BCAA.
The experimental procedures and identified markers will serve as the scientific basis to develop more efficient and rapid methods for determination of BCAA imbalance. This will contribute to improvements in pig health and performance and will alleviate their environmental impact.
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